Staining of PVDF after transfer?
Dr Dave Parcej
parcej at biophys.mpg.de
Wed Mar 4 02:46:17 EST 1998
In article <parcej-0303981541170001 at mac-kb5.biophys.mpg.de>,
parcej at biophys.mpg.de (Dr Dave Parcej) wrote:
Thats 0.01% sulphorhodamine!!!!!!
> Its odd that Ponceau S didnt work, but maybe youd like to try this:
> Briefly dip the blot in a 0.01%(w/v) prepared in 30% methanol. I think (ie
> you should test it with an unimportant sample first!) that you can destain
> with either water or methanol. We used this for preparing samples for
> Heres the reference:
> Pappin et al (1990) Analyt Biochem. 187, 10-19
> Let me know how it works!
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