Staining of PVDF after transfer?

Dr Dave Parcej parcej at biophys.mpg.de
Wed Mar 4 02:46:17 EST 1998


In article <parcej-0303981541170001 at mac-kb5.biophys.mpg.de>,
parcej at biophys.mpg.de (Dr Dave Parcej) wrote:

Thats 0.01% sulphorhodamine!!!!!!

> Dirk
> Its odd that Ponceau S didnt work, but maybe youd like to try this:
> Briefly dip the blot in a 0.01%(w/v) prepared in 30% methanol. I think (ie
> you should test it with an unimportant sample first!) that you can destain
> with either water or methanol. We used this for preparing samples for
> sequencing.
> Heres the reference:
> Pappin et al (1990) Analyt Biochem. 187, 10-19
> Let me know how it works!
> Dave
> 
> 
>



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