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Staining of PVDF after transfer?

Dr Dave Parcej parcej at biophys.mpg.de
Tue Mar 3 09:41:17 EST 1998

Its odd that Ponceau S didnt work, but maybe youd like to try this:
Briefly dip the blot in a 0.01%(w/v) prepared in 30% methanol. I think (ie
you should test it with an unimportant sample first!) that you can destain
with either water or methanol. We used this for preparing samples for
Heres the reference:
Pappin et al (1990) Analyt Biochem. 187, 10-19
Let me know how it works!

article <34FC0E1D.D33FCCE3 at mpih-frankfurt.mpg.de>,
Junghans at mpih-frankfurt.mpg.de wrote:

> Hi
> I have a problem with staining of PVDF-membranes after western-blot
> transfer.
> How can I stain the marker or in general my proteins before
> immunodetection. I tried PonceauS but this didn't work even after adding
> some methanol the result was not very promising, because only very
> strong bands were recognized.
> Does anybody know a more sensitive method?
> Thanks, Dirk Junghans
> --
> **************************************
> Dirk Junghans
> Max-Planck-Institut f. Hirnforschung
> Abt. Neurochemie
> Deutschordenstr. 46
> 60528 Frankfurt am Main
> e-mail: junghans at mpih-frankfurt.mpg.de
> Tel. ++49 - 69-96769-457
> Fax. ++49 - 69-96769-441

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