Staining of PVDF after transfer?

Dr Dave Parcej parcej at biophys.mpg.de
Tue Mar 3 09:41:17 EST 1998


Dirk
Its odd that Ponceau S didnt work, but maybe youd like to try this:
Briefly dip the blot in a 0.01%(w/v) prepared in 30% methanol. I think (ie
you should test it with an unimportant sample first!) that you can destain
with either water or methanol. We used this for preparing samples for
sequencing.
Heres the reference:
Pappin et al (1990) Analyt Biochem. 187, 10-19
Let me know how it works!
Dave


article <34FC0E1D.D33FCCE3 at mpih-frankfurt.mpg.de>,
Junghans at mpih-frankfurt.mpg.de wrote:

> Hi
> I have a problem with staining of PVDF-membranes after western-blot
> transfer.
> How can I stain the marker or in general my proteins before
> immunodetection. I tried PonceauS but this didn't work even after adding
> some methanol the result was not very promising, because only very
> strong bands were recognized.
> Does anybody know a more sensitive method?
> Thanks, Dirk Junghans
> --
> **************************************
> Dirk Junghans
> Max-Planck-Institut f. Hirnforschung
> Abt. Neurochemie
> Deutschordenstr. 46
> 60528 Frankfurt am Main
> 
> e-mail: junghans at mpih-frankfurt.mpg.de
> Tel. ++49 - 69-96769-457
> Fax. ++49 - 69-96769-441
>



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