ideal yeast expression system for (plant) protein expression

Benan Dincturk knben at ttacs.ttu.edu
Thu Mar 5 12:47:41 EST 1998


Hello netters

E. coli expression does not seem to work for my huge protein. So while I am
playing with E. coli vectors, I plan to start with yeast expression. Who
has experience with yeast? Can you suggest some vectors with strong
promoters? What are the important points in yeast transformation? I know
that is the thoughest part usually. I am a beginner so every suggestion
will be appreciated. Thank you all.

Benan




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