Ligand Binding

Marco Bocola bocola at mailer.uni-marburg.de
Thu Mar 5 12:35:40 EST 1998



Dr E. Buxbaum wrote:

> Louis Hom wrote:
> >
> > Basic question:
> >         Is there an activation energy associated with ligand binding?
>
> Yes, there is, and it is easy to measure. Simply determine the Kd at
> various temperatures and plot ln(Kd) vs the reciprocal temperature
> (absolute temperature, i.e. in Kelvin). If certain conditions are met,
> then the data points will be on a straight line, from the slope of which
> you can calculate the activation energy.

 under the assumption that the heat capacity of the system, the local
environment and solvation and furthermore the conformation and folding of
the protein is temperature independent......




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