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Staining of PVDF after transfer?

Mara Casar casar at niehs.nih.gov
Thu Mar 5 11:56:07 EST 1998

You can also stain a blot AFTER doing your Western (ECL, HRP, etc.)
detection.  Use Coomassie G-250, not R-250 - R is the one you use for
gels.  Make up the stain and destains the same way as you would for
gels, but then stain your blot for just a few minutes.  It's not
reversible, though, so it's best to do it last.


Junghans wrote:
> Hi
> I have a problem with staining of PVDF-membranes after western-blot
> transfer.
> How can I stain the marker or in general my proteins before
> immunodetection. I tried PonceauS but this didn't work even after adding
> some methanol the result was not very promising, because only very
> strong bands were recognized.
> Does anybody know a more sensitive method?
> Thanks, Dirk Junghans
> --
> **************************************
> Dirk Junghans
> Max-Planck-Institut f. Hirnforschung
> Abt. Neurochemie
> Deutschordenstr. 46
> 60528 Frankfurt am Main
> e-mail: junghans at mpih-frankfurt.mpg.de
> Tel. ++49 - 69-96769-457
> Fax. ++49 - 69-96769-441

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