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How can I isolate targets of proteins (cytosolic) ?

noone noone at cancer.bham.ac.uk
Fri Mar 6 09:55:10 EST 1998


In article <6doqrc$mdo at uni2f.unige.ch>, "Andreas Mühlemann"
<Andreas.Muehlemann at biochem.unige.ch> wrote:

> Hello !
> I'm working on a neuronal cytosolic protein and I'd like to
> isolate its unknown target in vivo.
> Any ideas on the approach I could choose ?
> 
> Tanx.
> Andreas.

Hi Andreas,

I think some of the usual approaches are

Immunoprecipitation
Yeast two hybrid
Expression of tagged protein and pull-out assay
BiaCore


Peter



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