Disappearing His-tagged proteins

Rob mbzrl at mbn1.biochem.nottingham.ac.uk
Fri Mar 6 06:50:18 EST 1998


Hi
Has anyone experienced his-tagged proteins apparently 'disappearing' on
FPLC gel filtration columns (eg Superose 6 or 12 columns). I assume they're
coming out of solution, but it's happened now for the last 2 his-tagged
proteins I've tried to purify but doesn't happen for the GST-tagged
equivalents.... Very strange!
Thanks in advance
Rob



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