His-tagged protein and SDS-PAGE migration
Arnoud van Vliet
arnoud at vanvliet.u-net.com
Tue Mar 10 11:43:37 EST 1998
I don't know the system, but if it is a 6-His system, then it can run
a bit higher on SDS-PAGE. My 10 and 14 kDa proteins (low histidine
content) ran at 16 and 20 kDa, respectively. However, there are
exceptions to the rule, as a third protein I expressed (high histidine
content) ran at the expected MW.
So summarizing: yes, up to 6 kDa higher (consult the QiaExpress manual
Hope this helps
On Tue, 10 Mar 1998 13:33:45 GMT, ct133 at columbia.edu wrote:
>Does anybody know whether his-tagged protein expressed from pRSET
>vector (Invitrogen) can migrate aberrantly on SDS-PAGE?
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