nondenaturating PAGE

Silke Beismann sbeisma at Uni-MolGen.gwdg.de
Thu Mar 19 05:27:34 EST 1998


Who can give me some hints?
I want to carry out a nondenaturating PAGE. Neither me nor anyone else
in my lab has ever done this before.
What is the best buffer system? Can I cast the gel in the same slab gel
casting device used for SDS-gels?
What picture would one expect to find if I load the gel with two
proteins I expect to form a complex? One is 23 kDa with a pI of 6.79 and
a net charge of -2, the other one is 28 kDa with a pI of 5.06 and a net
charge of -8.
I will be happy about any help I can get!
Silke



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