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Peptide Mapping/Asp-N protease

olivier Coqueret olivier at lan1.molonc.mcgill.ca
Thu Mar 19 17:03:11 EST 1998


Hi,
I'am trying to do peptide mapping using Asp-N protease. Has anyone
worked out the optimal conditions? Reaction buffer, pH and temperature,
reaction time and enzyme quantity?
Many thanks
Olivier



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