p_hufnagel at YAHOO.COM
Sat Mar 21 02:04:32 EST 1998
Who has experiences with enterokinase?
I want to use it for cleaving a fusion protein and have some questions:
Is enterokinase really specific for DDDDK?
Does enterokinase modifiy the new amino terminus after cleavage?
Are there any "special conditions" for the cleavage? E.g. temperature,
molar ratio, ...
Thanks a lot.
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