enterokinase

Peter Hufnagel p_hufnagel at YAHOO.COM
Sat Mar 21 02:04:32 EST 1998


Who has experiences with enterokinase?
I want to use it for cleaving a fusion protein and have some questions:

Is enterokinase really specific for DDDDK?

Does enterokinase modifiy the new amino terminus after cleavage?

Are there any "special conditions" for the cleavage? E.g. temperature,
molar ratio, ...

Thanks a lot.
Peter
*****








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