His-tagged protein and SDS-PAGE migration

James Bassuk bassuk at u.washington.edu
Thu Mar 26 01:07:20 EST 1998


nonsense.  his-tag won't by itself cause nothing more than an increase
in MW of 600.

but, being a recombinant protein, all kinds of screwy things can happen.
your question is too general because every protein would have an
emperical answer.

Jim Bassuk
U Washington
Seattle


On Tue, 10 Mar 1998 ct133 at columbia.edu wrote:

> Hi,
> 
> Does anybody know whether his-tagged protein expressed from pRSET
> vector (Invitrogen) can migrate aberrantly on SDS-PAGE? 
> 
> Thanks,
> 
> C. Tunyaplin
> 
> 




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