His-tagged protein and SDS-PAGE migration
bassuk at u.washington.edu
Thu Mar 26 01:07:20 EST 1998
nonsense. his-tag won't by itself cause nothing more than an increase
in MW of 600.
but, being a recombinant protein, all kinds of screwy things can happen.
your question is too general because every protein would have an
On Tue, 10 Mar 1998 ct133 at columbia.edu wrote:
> Does anybody know whether his-tagged protein expressed from pRSET
> vector (Invitrogen) can migrate aberrantly on SDS-PAGE?
> C. Tunyaplin
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