His-tagged protein and SDS-PAGE migration

[James Bassuk]

nonsense.  his-tag won't by itself cause nothing more than an increase
in MW of 600.

but, being a recombinant protein, all kinds of screwy things can happen.
your question is too general because every protein would have an
emperical answer.

Jim Bassuk
U Washington
Seattle


On Tue, 10 Mar 1998 ct133 at columbia.edu wrote:

> Hi,
> 
> Does anybody know whether his-tagged protein expressed from pRSET
> vector (Invitrogen) can migrate aberrantly on SDS-PAGE? 
> 
> Thanks,
> 
> C. Tunyaplin
> 
>