u-PA purification

Jeremy M. Murray bmbjmm at bmb.leeds.ac.uk
Thu Mar 26 12:22:13 EST 1998


Fei WANG wrote:

> I plan to purify u-PA and have a problem to solve. In the last step, I
> should to concentrate the u-PA, does anyone know the best way to concentrate
> with less-inactivation and with less-convert from one-chain form to
> two-chain form. Please help me.

 Hi Fei WANG
wot is u-PA?
if it is a protein the dimerisation might be concentration dependent anyways?
jez

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Jeremy Murray                            MAIL: bmbjmm at bmb.leeds.ac.uk
Biochemistry & Molecular Biology         TEL:  +44 113 233 2591
Leeds University, LEEDS LS2 9JT          FAX:  +44 113 233 3167
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