IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

hydroxylamine cleavage in GuHCl

Scott McMahan mcmahan at oncology.wisc.edu
Thu Mar 26 17:24:46 EST 1998

In article <Pine.WNT.3.95.980326112840.-65629A-100000 at tonto.scs.unr.edu>,
"Judith A. Airey" <jaairey at med.unr.edu> wrote:

:After stirring a couple of mins adjust to pH 9 using LiOH (or occasionally
:HCl).  Be careful when you get close to pH 9 since there is little
:buffering capacity at this pH.  Use immediately.

I threw in some ethanolamine to use as a buffering agent.  It made things a
lot easier.

                                         Scott McMahan
                                         mcmahan at oncology.wisc.edu

More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net