His-tagged protein and SDS-PAGE migration
hgtsei at med-rz.uni-sb.de
Fri Mar 27 11:32:10 EST 1998
<Pine.OSF.3.96.980325220537.21753J-100000 at saul9.u.washington.edu>, James
Bassuk <bassuk at u.washington.edu> wrote:
> nonsense. his-tag won't by itself cause nothing more than an increase
> in MW of 600.
> but, being a recombinant protein, all kinds of screwy things can happen.
> your question is too general because every protein would have an
> emperical answer.
> Jim Bassuk
> U Washington
> On Tue, 10 Mar 1998 ct133 at columbia.edu wrote:
> > Hi,
> > Does anybody know whether his-tagged protein expressed from pRSET
> > vector (Invitrogen) can migrate aberrantly on SDS-PAGE?
> > Thanks,
> > C. Tunyaplin
It is NOT nonsense. I also experienced with several different proteins
that adding a his6-tag can cause the protein to migrate at a higher
molecular mass in SDS-PAGE. I estimate the difference to about 2-3
University of Saarland,
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