Getting a good thrombin, as the others have stated, is key to success.
In our lab, we have found that the best and most specific thrombin is
the human isolate offered by Sigma (T-6759). We have performed
incubations at RT of up to 30h without significant secondary degradation
(in cleavages of 1.5kD peptide from GST).
Good luck, Randy
Randall C Willis, Researcher
Biochemistry Research, Hospital for Sick Children
3421-555 University Ave
M5G 1X8 CANADA
416-813-5933 (ph) -5022 (fax)
willis at gandalf.psf.sickkids.on.ca
Randall C Willis, Publisher
Aliquotes: A Journal Of Molecular and Biochemical
Humour and Information
58 Balfour Ave.
M4C 1T6 CANADA
416-691-2921 (ph) 416-813-5022 (fax)
roger at xybercom.net