Thrombin sources (was: pgex expression)

[Randall C Willis]

Justin,

Getting a good thrombin, as the others have stated, is key to success. 
In our lab, we have found that the best and most specific thrombin is
the human isolate offered by Sigma (T-6759).  We have performed
incubations at RT of up to 30h without significant secondary degradation
(in cleavages of 1.5kD peptide from GST).

Good luck, Randy

Randall C Willis, Researcher
Biochemistry Research, Hospital for Sick Children
3421-555 University Ave
Toronto, ON
M5G 1X8  CANADA

416-813-5933 (ph)  -5022 (fax)
willis at gandalf.psf.sickkids.on.ca


Randall C Willis, Publisher
Aliquotes Press
Aliquotes: A Journal Of Molecular and Biochemical
              Humour and Information
58 Balfour Ave.
Toronto, ON
M4C 1T6  CANADA

416-691-2921 (ph)  416-813-5022 (fax)
roger at xybercom.net