How to fix bound 'DNA binding proteins' to DNA?

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Thu May 14 13:36:23 EST 1998


In article <355A50BB.2781 at salt2.med.harvard.edu>, Jong Park <jong at salt2.med.harvard.edu> wrote:
:Hi,
:
:I wonder anybody can tell me how to fix any DNA binding
:proteins to DNA. Any possible methods are welcome.
:Crosslinking, increasing affinity by adding some reagents,
:chemically modifying etc. Any comments are welcome.
:
:Thanks very much and I am looking forward to your
:replies.
:I appreciate sending me copies of reply to my email
:address as well.

This really depends on what you need it for afterwards.
UV should cross-link just fine.

        - Dima



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