IgG-like proteins in yeast?

Rick00100 rick00100 at aol.com
Sun May 17 08:33:44 EST 1998


Hi group,

I'm doing Western to study genes transformed into yeast.  Funny thing was that
I got the same banding patterns in transformed and untransformed strains.  So
the other day I probed my yeast lysate with secondary antibodies only.  What do
you know, I have the same bands!  I tried a few secondary Abs, including
monoclonal mouse anti-Rabbit IgG!  Guess I'm going to do immunoprecipitation
before Western next time, but I'm wondering if anybody has any comment on this?

Thanks,
Red Lowe



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