IgG-like proteins in yeast?
Rick00100
rick00100 at aol.com
Sun May 17 08:33:44 EST 1998
Hi group,
I'm doing Western to study genes transformed into yeast. Funny thing was that
I got the same banding patterns in transformed and untransformed strains. So
the other day I probed my yeast lysate with secondary antibodies only. What do
you know, I have the same bands! I tried a few secondary Abs, including
monoclonal mouse anti-Rabbit IgG! Guess I'm going to do immunoprecipitation
before Western next time, but I'm wondering if anybody has any comment on this?
Thanks,
Red Lowe
More information about the Proteins
mailing list