DE-52 Columns

Leanne Allhouse leanne.allhouse at physiol.ox.ac.uk
Wed May 20 06:51:58 EST 1998


I was once shown how to purify barnacle tnc proteins using DE-52 columns
using the buffer 6M urea, 50mM Tris-HCl, 1mM EDTA,1mM DTT but was
wondering if anyone can tell me why it is important to measure the
conductivity of the column buffer, loaded sample and eluted fractions?

Thanks
Leanne



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