?Criterion for right conformation?

Dr E. Buxbaum EB15 at le.ac.uk
Wed May 20 19:05:02 EST 1998


Jongrak Kittiworakarn wrote:

> I have expressed my peptide, a truncated fragment of a protein, in
> insoluble form, then I refolded it into water soluble peptide. 
> Anyone would please give me general criterion to determind whether the
> refolded protein
> adopted the propered conformation? 

2D NMR would be the most rigorous proof. You'll have to get into contact
with somebody who has the equipment and expertise to do this.



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