DE-52 Columns

Laura Moen lmoen at odu.edu
Wed May 20 11:30:49 EST 1998


Leanne,
   DE-52 is an anion exchange resin.  Like other ion exchange resins, that
means that ionic strength is an important factor in protein interactions
with the column matrix.  If you want to know more about the use of ion
exchange resins in protein purification and other things, you might look in
Scopes book on Protein Purification.  Also, I suspect other individuals in
the laboratory where you are working can be of assistance.  And, there may
even be a technical information sheet from the manufacturer of the resin
which can provide additional info.  Hope this helps.  Laura



Leanne Allhouse wrote:

> I was once shown how to purify barnacle tnc proteins using DE-52 columns
> using the buffer 6M urea, 50mM Tris-HCl, 1mM EDTA,1mM DTT but was
> wondering if anyone can tell me why it is important to measure the
> conductivity of the column buffer, loaded sample and eluted fractions?
>
> Thanks
> Leanne






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