Mono-Q/ATP

Rob mbzrl at mbn1.biochem.nottingham.ac.uk
Wed May 27 09:06:08 EST 1998


Hi
Does anyone have experience of using ATP-containing buffers with Mono-Q (or
equivalent) FPLC columns. In particular, am I correct in assuming that ATP
binds the column and comes off as a peak around 0.15-0.20M NaCl (in 50mM
Tris pH7.5)?
Thanks in advance
Rob



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