protein-protein interactions

Matthew Hicks baps9 at central.susx.ac.uk
Tue Oct 6 11:10:56 EST 1998


The best method in my opinion would be analytical ultracentrifugation
(AUC) - look at the Beckman homepage on the WWW or see the National
Hydrodynamics Centre homepage (Nottingham University - UK). This method
can potentially give association constants in a native-like buffer.
X-linking would show interaction but not much about the tightness of
binding - also you _may_ have to use 'weird' buffer conditions which may
be required for chemical
modification of the proteins.  If you cannot get
access  to the kit then how about using size exclusion chromatography to
see if your samples coelute at a molecular weight above that of the
individual proteins - this would show interaction (but not as well as
AUC!)
Good Luck
Matt Hicks
University of Sussex (UK)





More information about the Proteins mailing list