Native immunoprecipitation

[Gerrit Bouw]

Dear everyone,

I am looking for a method to immunoprecipitate without breaking all
protein-protein interactions. The complex I want to precipitate is a
heterotetrameric complex, bound
by coiled-coil interactions. I am looking for a procedure to
immunoprecipitate without
addition of SDS and other detergents...... The proteins are type I
transmembrane
proteins and have to be resolved from the membrane.

Could anyone help me with this???

Thanx in advance..

Gerrit Bouw