s35 labeling of proteins

Pinky Agbuya moenlab at CYAN.SCI1.ODU.EDU
Fri Oct 9 08:29:26 EST 1998


We are trying label a plasmid-encoded protein under the control of a T7
promoter.  The protocol calls for an amino acid mixure to be
supplemented in the minimal media.  Does anyone know the preferred
concentration of amino acids in minimal media for this procedure?



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