?Why alkaline pH in protein refold?

Roger Murphy murphy_r at licre.ludwig.edu.au
Wed Oct 14 23:21:34 EST 1998

In article <3624020E.C612EAA5 at student.mahidol.ac.th>, g3937506 at STUDENT1.MAHIDOL.AC.TH (Jongrak Kittiworakarn) wrote:
>Dear all:
>Is it neccessary to refold a protein in alkaline pH?
>Does this prevent any chemical modification ?
>Thanks for all suggestion.
>-----------------------------------Mr. Jongrak Kittiworakarn

Hi Jongrak,

The reason for protein refolding at slightly alkaline pH is that disulfide 
bridge exchange is pH dependant, and is faster at alkaline pH.  Thus you can 
get rapid making and breaking of disulphide  bridges until the 
thermodynamically most stable form of your protein is formed. 

Hope this helps.


Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg,  Vic. 3084

Tel  61-3-94965463
Fax  61-3-94965436
Email murphy_r at licre.ludwig.edu.au

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