?Why alkaline pH in protein refold?
murphy_r at licre.ludwig.edu.au
Wed Oct 14 23:21:34 EST 1998
In article <3624020E.C612EAA5 at student.mahidol.ac.th>, g3937506 at STUDENT1.MAHIDOL.AC.TH (Jongrak Kittiworakarn) wrote:
>Is it neccessary to refold a protein in alkaline pH?
>Does this prevent any chemical modification ?
>Thanks for all suggestion.
>-----------------------------------Mr. Jongrak Kittiworakarn
The reason for protein refolding at slightly alkaline pH is that disulfide
bridge exchange is pH dependant, and is faster at alkaline pH. Thus you can
get rapid making and breaking of disulphide bridges until the
thermodynamically most stable form of your protein is formed.
Hope this helps.
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Heidelberg, Vic. 3084
Email murphy_r at licre.ludwig.edu.au
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