medium of deuteriun-oxide

[Jan Aelen]

Dear Netters,

I want to express my protein in LB dissolved in deuterium-oxide instead
of normal water.
The host strain is BL21/DE3.
Has anyone experience about the growth of the cells in this medium?

Thanks

--
Mr.J.M.A.Aelen
N.S.R.Center
Dept.Biofysical of Biochemistry
Faculty of  Science
Toernooiveld 1
6525 ED
Nijmegen
The Netherlands
Tel.31.24653178
Fax.31.24652112
e-mail janal at nmr.kun.nl