protein folding

camoin luc camoin at icgm.cochin.inserm.fr
Fri Oct 16 07:22:56 EST 1998



Dear Netters,

   I'am looking advice about protein refolding.

   I'am not familiar with these techniques so all advices will be welcome.

   We tried to fold correctly a 20kDa recombinant protein expressed in
Ecoli. This protein was found in inclusion bodies.
   Correct folding is necessary to allow its disulfide bridge to be restored.

   Does anyone can send me a detailed protocol to refold a protein?

   Thanks in advance



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