blotting fixed gels
hroychow at NMSU.EDU
Fri Oct 16 17:43:09 EST 1998
At 07:05 PM 10/16/98 GMT, irabinov at bidmc.harvard.edu wrote:
>Does anyone know if it is possible to blot a dried gel (PAGE)that
>was fixed in Coomasie/destain?
>I'd appreciate any suggestion or method.
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Here is something that I know is possible: One can electrophorese
out polypeptides fixed and stained (CBB-R) in acryl. gels. This is a routine
method of obtaining antigens for some.
So, it may be possible to equilibrate such gels in Towbin's and
transfer the content to PVDF using the usual western transfer set up.
Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu
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