Nuclear localisation

A Al-dukhyil MBQ96AA at sheffield.ac.uk
Mon Oct 19 06:12:44 EST 1998


Hi,
I've got a fusion of Green fluorescent protein to Nuclear localisation 
sequence in a vector. When I transfect this vector to mammalian cell 
lines I get a mixtures of transfected cells, some of them have GFP in the 
nucleos and the others have GFP in the whole cell. Is it normal to get a 
result like this?
Thanking in anticipation



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