Amylase assay

Hao Xiao haoxiao at
Tue Oct 27 18:12:33 EST 1998

Using dye-labeled starch. I think it is azo-dye stain starch
The blue dye starch is particulate, when amylase hydrolyze the starch, the
dye (Abs 490 nm?) will be released into the solution. The amount of dye in
the solution (UV Abs) is proportional to the amount of specific activity of


Lars Komorowski wrote in message
<70vcd5$h63$1 at>...
>Who can tell me a specific alpha-amylase assay from which to determine the
>specific activity of the enzyme ?

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