Help on plant protein extraction.
baslee at kabelfoon.nl
Wed Oct 28 15:46:45 EST 1998
Wenjin Yu wrote:
> Dear friends,
> I'm going to extract total proteins from plant tissues but don't
> want to use denature buffer such as SDS-sample buffer. Could you
> recommend a buffer for me, which can obtain a maximum amount of protein
> but keep the protein native? Please send your response to me e-mail
> address: wyu at uoguelph.ca.
> Thanks in advance.
> Wenjin Yu
My first guess would be a buffer at the pH of your sample (I gues pH 4-5
eg malonic or acetic acid buffers) containing oxidation repressing
compounds. (eg Methionine or Vit. C)
---=== Bassie ===---
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