Help on plant protein extraction.

[Bassie]

Wenjin Yu wrote:
> 
> Dear friends,
>         I'm going to extract total proteins from plant tissues but don't
> want to use denature buffer such as SDS-sample buffer.  Could you
> recommend a buffer for me, which can obtain a maximum amount of protein
> but keep the protein native?  Please send your response to me e-mail
> address: wyu at uoguelph.ca.
> Thanks in advance.
> 
> Wenjin Yu

Well Wenjin,

My first guess would be a buffer at the pH of your sample (I gues pH 4-5
eg malonic or acetic acid buffers) containing oxidation repressing
compounds. (eg Methionine or Vit. C)

---=== Bassie ===---