Detection of activated G proteins

Tom Vink t.vink at med.uu.nl
Thu Oct 29 09:19:11 EST 1998


Hi all,

I am trying to detect to which G protein a GPCR is coupled and want to
label the G-protein with radioactive GTP. Which type of label is the
best. I think the photolabel technique is the best but the photolabel
is not commercially available ? Any ideas or protocols are welcome.

Thanks Tom




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