Protease digestions

Kresten kresten at my-deja.com
Mon Aug 2 05:34:25 EST 1999


In article <7o3rvm$c91$1 at nnrp1.deja.com>,
  Kresten <kresten at my-deja.com> wrote:
>
>
> > Please send /or post your favorite protease digestion portocols to
me
>
> I have recently used trypsin for detection of a post-translational
> modification.
>
> I used:
> 1% w/w trypsin in a 50 mM (NH4)(HCO3) pH 8.2
>
> I stopped the reaction with a small amount of HCl and analysed
directly
> on a MALDI mass-spectrometer (by taken out samples I did a time course
> analysis). This may of course not be the best way for you since your
> protein is approx. 3 times larger

sorry, that should have been *30* times larger
kresten

> than mine and you modification a bit
> smaller but you could try. You could also try to purify the tryptic
> peptides on a HPLC.
>
> HTH
> Kresten
>
> >
> > Thanks in advance
> >
> > Dirk
> >
>
> --
> The address kresten at my-dejanews.com is for
> spambots only. Please mail me at LysLeuLeu at crc.dk
> transforming the pre at -part into my initials.
> Kresten Lindorff Larsen, Dept. Yeast Genetics
>
> Sent via Deja.com http://www.deja.com/
> Share what you know. Learn what you don't.
>

--
The address kresten at my-dejanews.com is for
spambots only. Please mail me at LysLeuLeu at crc.dk
transforming the pre at -part into my initials.
Kresten Lindorff Larsen, Dept. Yeast Genetics


Sent via Deja.com http://www.deja.com/
Share what you know. Learn what you don't.



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