electrophoresis advice

Ossama B Kashlan okashlan at mail2.sas.upenn.edu
Tue Aug 3 12:36:54 EST 1999


I'm trying to visualize a crosslinked protein on a polyacrylamide gel, and
am having some difficulty with sharpness with the heavy bands.  My
expected products have masses between 90 kD and 540 kD.  I'm allowing the
90 kD monomer to run to the bottom of the gel.  I've run a 4% 20 cm x 20
cm x 1.5 mm gel with a 4% stacking gel.  Any suggestions as to how to
sharpen an expected 540 kD band? 

Thank you,
Ossama Kashlan
University of Pennsylvania
Department of Chemistry
Box 233
231 S. 34th St.
Philadelphia, PA  19104
okashlan at mail.sas.upenn.edu
Lab: (215)898-2927
Fax: (215)898-2037




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