Properties of proteins purified in imidazole

Marc Saric marc.saric at
Thu Aug 12 05:28:48 EST 1999

frank rozsa schrieb:
> 1. Does imidazole interfere with SDS-PAGE? If not, can I use the blot for
>    subsequent western blots?
No experience with Western Blotting, but it should work for normal
SDS-Gels (no problems here with a wider range of soluble proteins
expressed in E.coli).


Marc Saric

Max-Planck-Institut für molekulare Physiologie
Otto-Hahn-Strasse 11
44227 Dortmund

phone: +49 231 133 2168

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