about 'mini-protean 2' by bio-rad

[Joo Yun Hong]

hello!!

i'm using hydridoma supernatant to screen monoclonal antibody about
protein on a
membrane. since the amount of supernatant i can use is limited to a
small volume, i
used this apparatus named  'mini-protean2'  from the bio-rad. but it has
been giving
me a problem of leakage between  the wells. so i was wondering if anyone
could  tell
me about similar kind of apparatus using a membrane to screen multiple
samples of
hydridoma supernatant  or have  any idea of eliminating the leakage
problem.

                                                                   thank
you

joo         jhong1 at uic.edu