Signals for proteolysis

hernandez at POP.HORT.PURDUE.EDU hernandez at POP.HORT.PURDUE.EDU
Tue Aug 24 15:00:33 EST 1999


Dear all,

Is there a way of finding if a certain protein is likely to be short-lived 
in a cell just by looking at the amino acid sequence? When making a 
literature search, I have found many papers that mention an "N-end rule" 
but I haven't been able to figure out what that is beyond knowing that a 
Lys residue is needed for binding of ubiquitin. Besides, I got the 
impresion that these signalling domains can be both at the N or the 
C-terminus in a protein but I don't know if they are different. By the way, 
I work in yeast, in case this makes a great difference in the way the 
proteins are processed (as you can see, I haven't got much idea about all 
these processes, sorry!)
Could someone point me to a good www site for analysing my protein sequence 
and find if its turnover is likely to be rapid? Alternatively, anybody 
knows of a review that deals with domains in a protein that signal it for 
degradation?
I haven't got direct access to this newsgroup, so I'd appreciate if you 
could send any answers to my mail address:

hernandez at pop.hort.purdue.edu

Thanks!

Agustin Hernandez







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