iodoacetamide

Roger Murphy murphy_r at licre.ludwig.edu.au
Sun Aug 29 23:17:08 EST 1999


It derivatises free sulfhydryl groups - you reduce cys-cys disulfide bonds, 
react with iodoacetamide and they can't reform.

Standard procedure - look in any biochemistry text book.

Roger

In article <37c52585.486086 at news.univie.ac.at>, 
a8803349.nospam at unet.univie.ac.at (Martin Offterdinger) wrote:
>What is the purpose in using iodoacetamide for protein isolation. (I
>found it in a protocol) Does it inhibit (thiol-) proteases? Could it
>also be used to neutralize DTT or BME?
>Any comments welcome!
>Martin
>Martin Offterdinger
>Internal Med.I,Dept. Oncology
>University of Vienna
>Austria
>E-Mail:a8803349.nospam at unet.univie.ac.at
>(remove .nospam before mailing)


------------------------------------------------------------
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg,  Vic. 3084
Australia.

Tel  61-3-94965463
Fax  61-3-94965436
Email Roger.Murphy at Ludwig.edu.au



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