Western stripping

Martin Offterdinger martin.offterdinger at akh-wien.ac.at
Fri Dec 3 02:53:03 EST 1999


Neil McKenna <nmckenna at ottawa.com> schrieb in im Newsbeitrag:
3846E641.A1DA76B4 at ottawa.com...
> I have had difficulty finding an efficient way to strip
> chemiluminescent/fluorescent-detected Western blots. I recall using a
> B-mercaptoethanol solution and/or an SDS solution, but I can't recall
> the concentrations or the steps. Is there anyone out there who has a
> protocol?
>
> Many thanks
> Neil
Use 100mM B-mercaptoethanol, 2% SDS in 62,5mM Tris (pH=6.8), at 65°C for
30min. (I used 50°C and it was still good)
martin





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