Western stripping

Younes Bounhar ybounh at po-box.mcgill.ca
Tue Dec 7 12:58:37 EST 1999


you can also use 0.2 M NaOH for 5' at RT, it works just great. (don't leave
it any longer it will strip your antigen away too!!)


Younes


Neil McKenna <nmckenna at ottawa.com> wrote in message
news:3846E641.A1DA76B4 at ottawa.com...
> I have had difficulty finding an efficient way to strip
> chemiluminescent/fluorescent-detected Western blots. I recall using a
> B-mercaptoethanol solution and/or an SDS solution, but I can't recall
> the concentrations or the steps. Is there anyone out there who has a
> protocol?
>
> Many thanks
> Neil





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