GST dimers

Colin Rasmussen colin at pombe.usask.ca
Thu Dec 9 10:46:01 EST 1999



Alex Blais wrote:

> Hi,
>
> I am trying to purify a recombinant protein fused to GST overproduced in
> E.coli. I have some problems during the purification steps, and I
> hypothesised that homodimers formation mediated by the GST moiety could
> be the cause of the problem.

Two questions:

How do you know there are dimers?

Why does that pose a problem?

Colin




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