GST dimers

Ed Ralph e.ralph at *remove*biotech.cam.ac.uk
Fri Dec 17 10:38:09 EST 1999


Alex Blais <Alexandre.Blais at crchul.ulaval.ca> wrote in message
news:384E909B.313588B2 at crchul.ulaval.ca...
> Hi,
>
> I am trying to purify a recombinant protein fused to GST overproduced in
> E.coli. I have some problems during the purification steps, and I
> hypothesised that homodimers formation mediated by the GST moiety could
> be the cause of the problem.

You shouldn't get any problems from GST dimerisation.  It is probably
something else.  If you could supply details, we could troubleshoot.  I have
used GST fusions a fair bit, and haven't had any problems with dimerisation.

ed






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