Bradford protein determination; calibration curve
harold.taylor.remove-this at delete-this.uni-tuebingen.de
Sat Dec 18 11:26:46 EST 1999
PGegen at UKans.nolospamare.edu (Dr. Peter Gegenheimer) wrote:
>> You'll find that fitting your results to a hyperbolic function
>> i.e., Ext. = (protein * factor1) / (protein + factor2)
>> works quite well.
>If the relationship is not linear, you would first suspect that the reaction
>is not going to completion; that is, reagent concentration is limiting. This
>is especially true for the Bradford assay, which is (in principle) a simple
>[On the other hand, a curve of Abs vs [dye] must have a hyperbolic shape
>because its a binding isotherm. From this you could measure Kd but you
>wouldn't want to use it for a calibrations curve of dye concentration!]
Gee, I wish I'd put it that way ;-))
Thanks for cleaning up after me. Merry Christmas all from Germany!
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