Deglycosylation

DOCTORHIM at aol.com DOCTORHIM at aol.com
Tue Dec 21 12:33:12 EST 1999


Solubility of the deglycosylated protein is often a problem.  Some solutions:

1.  Increase the buffer concentration or salt concentration.  None of the 
Endo F enzymes including PNGase F are affected by even 0.7 M NaCl

2.  Decrease the protein concentration.

3.  Increase the temperature.  Why are you incubating at 4 C ?

4.  Change the pH (but don't go over pH 6 with Endo F2. 

You may be able to redissolve the precipitated protein.

Where are you getting Endo F2 from these days?
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