Enough Methonine?

Mike Mason M.Mason at Botany.uq.edu.au
Tue Feb 2 01:43:28 EST 1999


Hello all,

I am about to start an in vitro translation and would like to know two things:

1) I would prefer to use 35 S Methionine because it is readly available to me, 
however my protein contains only one Methionine in its sequence. Is this enough 
 to be able to visulise the protein on a SDS gel?

2) For plant proteins is it best to use wheat germ extract to translate the 
protein? I heard rabbit reticulocyte is sometimes better.

Thanks for your help in these matters,
Mike Mason




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