Insolubility of double-tagged protein

Ditlev Brodersen ding at imsb.au.dk
Mon Feb 1 11:28:52 EST 1999


Hi,

  we are having some trouble with a doubly-tagged (His in the N-terminus,
GST in the C-terminus) expressed in E. coli 834(DE3) pLysS which seems to
have very low solubility in our lysis buffer.

  I know this is a standard question, but what are the best things I can do
in order to optimise the amount of soluble protein?

Thanks in advance,

  Ditlev






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