Stability of protein in glycerol

Vladimir Rotrekl rotrekl at chemi.muni.cz
Sat Jan 9 10:39:19 EST 1999


Dear all,
   I have produced recently some mutant proteins, which are not really
examples of protein stability. But they are at least able to stay in
solution at 4C. The problem appeared when I tried to run native PAGE.
Samples precipitated after addition of glycerol(20%) loading buffer.
   My qustion is if (and if yes then how) it is possible to use other
compounds to prepare sample buffer for proteins (like in DNA 10% ficol or
sucrose).

Thanks a lot
    Vladimir





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