Transfecting sperm-cells

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Fri Jan 8 11:54:41 EST 1999


In article <76ss36$rqk$1 at wnnews.sci.kun.nl>, "Gerrit Bouw" <gbouw at sci.kun.nl> wrote:
:Dear everyone,
:
:Does anyone ever tried to transfect sperm-cells? If so, please could you
:give
:me some guidance on this?
:
:Gerrit
:
:

[what I don't unde4rstand is why this has been posted to *.proteins and 
not to methods and reagents...]

Yes, spermatozoa are just normal cells and can be lectroporated like any 
other. A 1 minute search in Medline:

Title   
    Sperm as a carrier to introduce an exogenous DNA fragment into the 
oocyte  ╕
    of Japanese abalone (Haliotis divorsicolor suportexta).                
    ╕
   Source                                                                  
    ╕
    Transgenic Research.  6(1):85-95, 1997 Jan.

Title   
    Nuclear internalization of foreign DNA by zebrafish spermatozoa and 
its    ╕
    enhancement by electroporation.                                        
    ╕
   Source                                                                  
    ╕
    Journal of Experimental Zoology.  274(2):121-9, 1996 Feb 1.


 Title  
    Sperm characteristics and outcome of human assisted fertilization by   
    ╕
    subzonal insemination and intracytoplasmic sperm injection [see 
comments]. ╕
   Source                                                                  
    ╕
    Fertility & Sterility.  59(4):826-35, 1993 Apr.

Title   
    Electroporation: a method for transferring genes into the gametes of   
    ╕
    zebrafish (Brachydanio rerio), channel catfish (Ictalurus punctatus), 
and  ╕
    common carp (Cyprinus carpio).                                         
    ╕
   Source                                                                  
    ╕
    Molecular Marine Biology & Biotechnology.  1(4-5):301-8, 1992 Aug-Oct.

 Title  
    Introducing foreign genes into fish eggs with electroporated sperm as 
a    ╕
    carrier.                                                               
    ╕
   Source                                                                  
    ╕
    Molecular Marine Biology & Biotechnology.  1(4-5):276-81, 1992 
Aug-Oct.


Title   
    Electroporation of bovine spermatozoa to carry foreign DNA in oocytes. 
    ╕
   Source                                                                  
    ╕
    Molecular Reproduction & Development.  29(1):6-15, 1991 May.


 Title  
    [The incorporation of macromolecules into the germ cells of male mice 
via  ╕
    electroporation and dimethyl sulfoxide]. [Russian]                     
    ╕
   Source                                                                  
    ╕
    Tsitologiia i Genetika.  24(3):3-7, 1990 May-Jun.


Hope this helps,

        - Dima




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