No protease activity in yeast extract!?
deleteme_cpeter at zedat.fu-berlin.de
Mon Jan 18 13:53:57 EST 1999
I am trying to determine protease activity in biological samples.
We have established an assay using Azocasein and the Protease-Kit
from Pierce. Using Trypsin and Papain as standards we get nice
calibration-curves with these assays. When we try a freshly made
yeast-extract as a supposedly positive sample (glass beads, no
additives to buffer (PO4, pH7.0)) we do not find the least bit of
Although this would be good news to everybody purifying yeast
proteins, I suppose something is really going wrong with the assays!
Who had similar experience or is using either assay successfully?
Thanks in advance...................................Christoph
PhD Student (Biotechnology)
cpeter at zedat dot fu-berlin dot de
>Alles wird gut!<(Beate)
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